MOLECULAR GENETIC EFFECTS OF SOIL IVANO-FRANKIVSK, DTTERMINED USING THE HETEROZYGOUS PLANTS NICOTIANA TABACUM SU / +
Keywords:
heterozygous plants Nicotiana tabacum Su / , the frequency of mutations, the intensity of soil contaminationAbstract
Modern methods of indicating the degree of mutagenic pollution based on the use of molecular genetic changes in transgenic plants germinated on samples of different soils. We analyzed the phenotype of heterozygous mutations manifestations plant Nicotiana tabacum Su / +, which displays different types of spots on the leaves, depending on the intensity of soil contamination. The appearance of spots on the leaves are the result of DNA damage in the locus Su. Plants grown in soils taken from five different districts of the city of Ivano-Frankivsk. Fields marked as clean: garden Taras Shevchenko and green economy (respectively plot №№ 1 and 2) and contaminated: microregion Pasichna, st. Pul'uj and park near "Ivano-Frankivsk National Medical University" (respectively sections №№ 3, 4 and 5). Installates the highest number and total area of the spots in the plants on the plot number 3. Analysis of mutation’s intensity index (IMI), which reflects the percentage of leaf area occupied by spots was low in plants grown on soil plot №№ 1 and 2, and the highest in plants from plots №№ 3, 4 and 5.
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